Detail

Basic Information

GTO ID GTD0008
Accession GSE229785
Altered gene miR-28
Gene xrefs Gene ID: 407020
Gene symbol: MIR28
Ensembl ID: ENSG00000207651
Treatment miR-28 mimics
Control GSM7177656;GSM7177657
Case GSM7177646;GSM7177647
Disease Diffuse Large B Cell Lymphoma
Disease information Disease group: Cancer
Definition: A B-cell lymphoma that is a cancer of B cells presenting as an aggressive tumour which can arise in virtually any part of the body.
Xref: DO:0050745 | EFO:0000403 | EFO:0001642 | EFO:1000157 | EFO:1000490 | ICD10:C83.3 | MONDO:0002571 | MONDO:0018905 | MSH:D016403 | NCI:C8851 | ORDO:544
Organism Homo sapiens
Tissue/Cell type Diffuse large B cell lymphoma tumor cell line
Characteristics time of ibrutinib treatment: 4h
Strategy RNA-seq
Platform GPL20301
Vector information
Vectorlentivirus
Vector Typelentiviral vector
Transgene Inserted Genethe miR-28 precursor sequence
Vector Production MethodThe miR-28 precursor sequence was cloned into the doxycycline-inducible pTRIPZ vector (Thermo scientific). Lentiviral supernatants were obtained 48 h after transfection of 293 T cells with packaging vectors (VSVG, Δ9.8) and pTRIPZ plasmids.

Differential genes

Volcano plot

−10−50510150123456
Up(1993)Down(1796)Not Sig(11795)Log2FC-Log10(adjusted P-value)-0.4350.4351.3

Heatmap

GSM7177656GSM7177657GSM7177646GSM7177647
−1−0.500.51Z-Score

Enriched regulations/functions/pathways




77.588.599.510immune response-regulating cell surface receptor signaling pathwaypositive regulation of T cell activationB cell activationregulation of T cell activationpositive regulation of leukocyte cell-cell adhesionpositive regulation of cell-cell adhesionregulation of leukocyte cell-cell adhesionpositive regulation of cell adhesionregulation of cell-cell adhesionleukocyte cell-cell adhesion
-Log10(P-value)

Differential cell type markers