| Vector | adeno-associated virus |
| Construct | rAAV8-anti-VEGF protein |
| Vector type | recombinant adeno-associated virus serotype 8 (rAAV8) vector |
| Transgene/Inserted gene | encoding a human VEGF receptor fusion protein |
| Regulatory element | the cytomegalovirus (CMV) early enhancer/chicken b-actin (CBA) ubiquitous promoter; a chicken b-actin intron; a rabbit b-globin poly (A) signal |
| Vector production method | HEK293 cells grown in 293Pro chemically defined 293M medium (BasalMedia) in suspension were triple transfected with pHelper, pAAV transgene (encoding pAAV-CBAanti-VEGF protein49), and pAAV Rep-Cap plasmids using FectoVIR-AAV (Polyplus) in a BIOSTAT STR200 bioreactor (Sartorius). |