| Vector | adeno-associated virus |
| Construct | AAV2.7m8-C11.CO.aflibercept |
| Vector type | recombinant, replication-deficient adeno-associated virus (AAV.7m8) vector |
| Transgene/Inserted gene | the codon-optimized aflibercept cDNA expression cassette, consisting of a recombinant chimeric protein comprising the VEGFA-binding portions of the extracellular domains of human VEGFA receptors 1 and 2 and the fragment crystallizable region (Fc) portion of human immunoglobulin (IgG1) |
| Regulatory element | the human cytomegalovirus (CMV) immediate-early enhancer and promoter; an enhancer element from the major late promoter (eMLP); a synthetic intron; a Kozak sequence driving expression of aflibercept; a human scaffold attachment region (SAR); the human growth hormone (GH) polyadenylation site |
| Vector production method | ADVM-022 was produced in the baculovirus expression system in Sf9 cells where two baculoviruses were used, one encoding the genes for AAV2 Rep and AAV2.7m8 Cap proteins, and the other encoding the vector genome carrying the codon-optimized aflibercept cDNA expression cassette. |