| Vector | lentivirus |
| Construct | scFv-4-1BB-CD3ζ |
| Transgene/Inserted gene | transmembrane CD8 domain; 4-1BB costimulatory domain; CD3z signaling domain |
| Vector production method | ARI-0001 cells were manufactured using the CliniMACS Prodigy system (Miltenyi). Approximately 100 × 106 T cells were stimulated to expand with anti-CD3 and anti-CD28 antibodies together with IL-7 and IL-15. 24 h after T cell activation, cells were transduced with a lentiviral vector containing the CAR gene construct at a multiplicity of infection of 10. Following expansion, that lasted from 7 to 10 days, the final ARI-0001 cell product was washed, cryopreserved, and tested for identity, potency, sterility, and adventitious agents. |
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