| Vector | lentivirus |
| Transgene/Inserted gene | Anti-human CD22 scFv; CD8α signal peptide; 4-1BB costimulatory; CD3ζ cytoplasmic region |
| Viral genome modification | Anti-human CD22 scFv or anti-human CD19 scFv was linked with the CD8α signal peptide, CD8α transmembrane domain, 4-1BB costimulatory and CD3ζ cytoplasmic region and assembled into lentiviral pCDH plasmids with GFP as the tag protein. |
| Vector production method | The lentiviruses were produced by transfecting HEK293T cells with CAR plasmids and packaging plasmids including PMD2.g (Invitrogen, USA), pMDLg/pRRE and pRSV-Rev (Biovector Science Lab, China). After isolation from the peripheral blood of healthy donors, T cells were cocultured with CD3/CD28 human T-activator Dynabeads (1 × 106/ml) (Gibco, USA) and recombinant human IL-2 (100-200 U/ml) (R&D, USA) in lymphocyte serum-free medium KBM581 (Corning, USA). T cells were transduced with lentiviruses for 24 h and incubated in medium changed every 2 days. |
| Additional feature | GFP |
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