Basic Information

Gene symbol WT1 Synonyms AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33 Type of gene protein-coding
Gene perturbation-related omics dataset PerturbAtlas | PertOrg
Description WT1 transcription factor

GTO ID GTC0571
Trial ID UMIN000011519
Disease Myelodysplastic Syndrome
Altered gene WT1
Therapeutic/Target gene Target gene
TherapyTCR-T cell
Treatment WT1 TCR-T cells
HLAHLA-A*24:02
PhasePhase1
Recruitment statusCompleted
TitleWT1-antigen specific TCR-gene transduced T lymphocytes transfer using MS3-WT1-siTCR vector for acute myelogeneous leukemia and myelodysplastic syndrome
Year2013
CountryJapan
Company sponsorMie University, Ehime University, Fujita Health University, Nagoya University, Takara Bio Inc
Vector information
Vectorretrovirus
ConstructpMS3-WT1-siTCR
Transgene/Inserted geneDNA encoding WT1235-243/HLA-A*24:02-specific TCR-α and TCR-β chains; interfering RNA constructs that specifically downregulate endogenous TCR
Vector production methodThe cells were stimulated with WT1 peptide. Brefeldin A was added, and they were then incubated with anti-CD8 monoclonal antibody (Becton Dickinson). The cells were stained intracellularly with anti-IFN-γ monoclonal antibody.

Clinical Result

Cohort 1
Administration route infusion
Dosage 2E8 cells, 1E9 cells, 5E9 cells
Donor type autologous
Pts 8
Age Adult, Older_Adult
Lymph depletion No
Adverse reactions 7/8(Skin redness and induration)
References PMID: 28860210

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