| Vector | lentivirus |
| Construct | scFv-4-1BB-CD3ζ |
| Transgene/Inserted gene | 86-amino-acid fragment from human CD8α, comprising a longer extracellular-domain fragment (55 amino acids versus 45 amino acids in the CD19-BBz(71) prototype) and a longer intracellular sequence (7 amino acids versus 3 amino acids; truncated, nonfunctional epidermal growth factor receptor (tEGFR) |
| Vector production method | PBMCs were collected from patients by leukapheresis. PBMCs were isolated and purified from leukapheresis products by Ficoll-Paque density-gradient centrifugation and were then seeded into flasks that had been coated with anti-CD3/anti-CD28 antibodies. PBMCs were cultured in X-VIVO 15 medium supplemented with IL-2. Two days after initial T cell activation, T cells were transduced with a lentivirus encoding CD19-BBz(86) CAR. Cells were expanded for an additional 11-13 d. |
| Additional feature | tEGFR |
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