Basic Information


Gene symbol tk

Gene symbol IL12A Synonyms CLMF, IL-12A, NFSK, NKSF1, P35 Type of gene protein-coding
Gene perturbation-related omics dataset PerturbAtlas | PertOrg
Description interleukin 12A

Gene symbol IL12B Synonyms CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2 Type of gene protein-coding
Description interleukin 12B



GTO ID GTC0266
Trial ID NCT02555397
Disease Prostate Cancer
Altered gene E1|IL-12A|IL-12B|yCD|SR39|tk
Therapeutic/Target gene Therapeutic gene
TherapyDNA oncolytic virus
Treatment AdV5-yCD/mutTKSR39rep-hIL12
PhasePhase1
Recruitment statusActive, Not Recruiting
TitlePhase 1 Trial of Oncolytic Adenovirus-Mediated Cytotoxic and Interleukin 12 Gene Therapy for Locally Recurrent Prostate Cancer After Definitive Radiotherapy
Year2015
CountryUnited States
Company sponsorHenry Ford Health System
Other ID(s)Prostate Cancer (9829)
Vector information
Vectoradenovirus
ConstructAd5- yCD/ mut TK SR39 rep -hIL-12
Vector typeadenoviral serotype 5 (Ad5) vector
Transgene/Inserted geneIL12A; IL12B; yCD; SR39; HSV-TK
Regulatory elementhuman cytomegalovirus (CMV) promoter
Vector production methodThe adenovirus was generated by calcium phosphate precipitation of the pCA14-yCD/mutTKSR39 and pBGH8k-hIL-12 plasmids linearized with PvuI and ClaI, respectively. The precipitated DNA was co-transfected in AD293 cells and then purified (Adenopure; Puresyn, Malvern, PA, USA).

Clinical Result

Cohort 1
Administration route intraprostatic injection
Dosage 1E10~1E12 vp
Pts 15
Age Adult, Older_Adult
Outcome Increase in IL-12, IFNγ, and CXCL10 was observed in 57%, 93%, and 79% patients, respectively. The PSA doubling time (PSADT) pre and post treatment has a median of 1.55 years vs 1.18 years.
Adverse reactions Chills; Mental status change; Dehydration; Diarrhea; Dyspnea; Fatigue; Fever; Flu-like symptoms; Nausea; Urinary frequency; Vomiting; Malaise; Pain(thigh, kidney, head, fingers); Anemia; Leukopenia; Lymphopenia; Neutropenia; Thrombocytopenia
References PMID: 37713401

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