| Vector | lentivirus |
| Construct | CAR-33-4-1BBζ-GFP |
| Transgene/Inserted gene | anti-CD33 scFv; human CD8α hinge and transmembrane domains; human 4-1BB and CD3ζ signaling domains |
| Vector production method | The cells were cultured in GT-T551 medium and activated by the addition of antihuman CD3 monoclonal antibody (Takara, Japan), recombinant human IL-2 (rhIL-2; Peprotech, Rocky Hill, NJ), and recombinant human interferon (IFN)-γ (Peprotech). The cells were transferred from the coated flasks to fresh flasks after 4 days. Fresh medium and 1,000 U/ml rhIL-2 were added every 3 days. The composition and purity were assessed by fluorescent-activated cell sorting on days 10-11. The cells were then harvested and termed as CART-33 cells. |
| Additional feature | GFP |
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