| Vector | lentivirus |
| Construct | pRRLSIN.cPPT.MSCV/GFP.wPRE |
| Transgene/Inserted gene | single open reading frame consisting of the TCR β and TCRα chains |
| Regulatory element | a 2A element from the porcine teschovirus (P2A) |
| Viral genome modification | The TCR donor C4 (TCRC4) gene construct was then inserted into the pRRLSIN.cPPT.MSCV/GFP.wPRE vector obtained from the lab of Richard Morgan after excising the green fluorescent protein (GFP) gene. |
| Vector production method | Cells were stimulated with the HLA-A2-restricted EBV (EBV280-288 BMLF1 [GLCTLVAML]) peptide. On day +1 and day +2 of the stimulation, the responding cells were transduced using the pRRLSIN.cPPT.MSCV/TCRC4βP2ATCRC4α.wPRE lentiviral vector at a target multiplicity-of-infection of ≤3. |