Vector | lentivirus |
Construct | NY-ESO-1c259T cells |
Transgene/Inserted gene | CTAG1B |
Regulatory element | EF1α promoter; a Woodchuck hepatitis virus post-translational regulatory element (WPRE) |
Vector production method | Vector was produced at the City of Hope (Duarte, CA) using transient transfection with four plasmids expressing the transfer vector, rev, VSV-G, and gag/pol, in 293T cells. Supernatant was collected at multiple time points, clarified, treated with Benzonase, and concentrated by tangential flow filtration and centrifugation. |
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