Basic Information

Gene symbol IGK Synonyms IGK@ Type of gene protein-coding
Description immunoglobulin kappa locus

GTO ID GTC0080
Trial ID NCT00881920
Disease Myeloma | Lymphoma | Leukemia
Altered gene IGK
Therapeutic/Target gene Target gene
TherapyCAR-T cell
Treatment kappa-CD28 T cells
Generation2nd
PhasePhase1
Recruitment statusRecruiting
TitleKappa-CD28 T Lymphocytes, Chronic Lymphocytic Leukemia, B-cell Lymphoma or Multiple Myeloma, CHARKALL
Year2009
CountryUnited States
Company sponsorBaylor College of Medicine
Other ID(s)H-23574-CHARKALL|CHARKALL
Vector information
Vectorretrovirus
ConstructscFv-CD28-CD3ζ
Transgene/Inserted genethe Ab targeting the κ light chain of human Ig produced by the CRL-1758 mouse hybridoma (ATCC) as a single chain (scFv); CD28 and ζ endodomains
Viral genome modificationA second-generation CAR construct containing both CD28 and ζ endodomains was generated by cloning the Ab targeting the κ light chain of human Ig produced by the CRL-1758 mouse hybridoma (ATCC) as a single chain (scFv). A spacer region derived from the human IgG1-CH2CH3 domains was cloned in-frame between the scFv and the signaling domains to facilitate detection by phenotypic analysis of the transgenic product. This cassette was then cloned into the SFG retroviral backbone.

Clinical Result

Cohort 1
Administration route intravenous infusion
Dosage 2E7 cells/m^2, 1E8 cells/m^2, 2E8 cells/m^2
Donor type Autologous
Pts 16
Age Adult, Older_Adult
Lymph depletion Yes
Outcome 2/16(CR); 1/16(PR); 6/16(SD)
References PMID: 27270177

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