| Vector | retrovirus |
| Transgene/Inserted gene | a TCR that recognizes the peptide SLLMWITQC, corresponding to residues 157 to 165 of NY-ESO-1 (NY-ESO-1:157-165) |
| Vector production method | Clinical grade good manufacturing practice retroviral supernatants were obtained from the National Gene Vector Laboratory at Indiana University. Patient pheresis samples were stimulated using 50 ng/mL of soluble anti-CD3 antibody (OKT3; Ortho-Biotech, Bridgewater, NJ) in the presence of 300 iU of recombinant IL-2, transduced with a retrovirus encoding the modified 1G4-α95:LY anti-NY-ESO-1 TCR using retronectin, kindly provided by Takara Bio (Otsu, Japan), and expanded further in vitro before adoptive transfer. |
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