NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,624.6
Mean read quality 14.7
Median read length 730.0
Median read quality 16.0
Number of reads 227,626.0
Read length N50 3,610.0
STDEV read length 2,298.0
Total bases 369,799,843.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 227620 (100.0%) 369.8Mb
>Q7 227613 (100.0%) 369.8Mb
>Q10 226486 (99.5%) 367.5Mb
>Q12 211842 (93.1%) 350.5Mb
>Q15 145922 (64.1%) 274.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.4 (124)
2 39.9 (158)
3 39.8 (22)
4 39.8 (26)
5 39.3 (249)
Top 5 longest reads and their mean basecall quality score
1 39426 (11.5)
2 32496 (17.9)
3 32199 (10.3)
4 31361 (18.1)
5 31203 (16.5)

Summary statistics after filtering

General summary
Mean read length 1,624.6
Mean read quality 14.7
Median read length 730.0
Median read quality 16.0
Number of reads 227,620.0
Read length N50 3,610.0
STDEV read length 2,298.0
Total bases 369,799,766.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 227614 (100.0%) 369.8Mb
>Q7 227607 (100.0%) 369.8Mb
>Q10 226480 (99.5%) 367.5Mb
>Q12 211836 (93.1%) 350.5Mb
>Q15 145916 (64.1%) 274.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.4 (124)
2 39.9 (158)
3 39.8 (22)
4 39.8 (26)
5 39.3 (249)
Top 5 longest reads and their mean basecall quality score
1 39426 (11.5)
2 32496 (17.9)
3 32199 (10.3)
4 31361 (18.1)
5 31203 (16.5)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot