NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,446.4
Mean read quality 14.8
Median read length 1,379.0
Median read quality 16.1
Number of reads 456,519.0
Read length N50 4,742.0
STDEV read length 2,798.7
Total bases 1,116,840,630.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 456514 (100.0%) 1116.8Mb
>Q7 456497 (100.0%) 1116.8Mb
>Q10 454181 (99.5%) 1110.3Mb
>Q12 427895 (93.7%) 1062.0Mb
>Q15 306800 (67.2%) 847.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.6 (23)
2 40.6 (90)
3 40.0 (56)
4 39.6 (33)
5 39.6 (58)
Top 5 longest reads and their mean basecall quality score
1 41246 (11.4)
2 38810 (9.6)
3 37315 (11.7)
4 34657 (18.3)
5 34333 (9.3)

Summary statistics after filtering

General summary
Mean read length 2,446.5
Mean read quality 14.8
Median read length 1,379.0
Median read quality 16.1
Number of reads 456,513.0
Read length N50 4,742.0
STDEV read length 2,798.7
Total bases 1,116,840,520.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 456508 (100.0%) 1116.8Mb
>Q7 456491 (100.0%) 1116.8Mb
>Q10 454175 (99.5%) 1110.3Mb
>Q12 427889 (93.7%) 1062.0Mb
>Q15 306794 (67.2%) 847.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.6 (23)
2 40.6 (90)
3 40.0 (56)
4 39.6 (33)
5 39.6 (58)
Top 5 longest reads and their mean basecall quality score
1 41246 (11.4)
2 38810 (9.6)
3 37315 (11.7)
4 34657 (18.3)
5 34333 (9.3)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot