NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,026.0
Mean read quality 14.2
Median read length 1,152.0
Median read quality 15.3
Number of reads 499,936.0
Read length N50 3,757.0
STDEV read length 2,486.6
Total bases 1,012,860,291.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 499935 (100.0%) 1012.9Mb
>Q7 499932 (100.0%) 1012.9Mb
>Q10 497370 (99.5%) 1006.1Mb
>Q12 464468 (92.9%) 957.5Mb
>Q15 282309 (56.5%) 643.3Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.7 (77)
2 39.5 (119)
3 37.6 (121)
4 37.3 (76)
5 36.1 (140)
Top 5 longest reads and their mean basecall quality score
1 56487 (11.3)
2 54106 (11.0)
3 51114 (12.7)
4 39950 (11.0)
5 39056 (15.4)

Summary statistics after filtering

General summary
Mean read length 2,026.0
Mean read quality 14.2
Median read length 1,152.0
Median read quality 15.3
Number of reads 499,934.0
Read length N50 3,757.0
STDEV read length 2,486.6
Total bases 1,012,860,261.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 499933 (100.0%) 1012.9Mb
>Q7 499930 (100.0%) 1012.9Mb
>Q10 497368 (99.5%) 1006.1Mb
>Q12 464466 (92.9%) 957.5Mb
>Q15 282307 (56.5%) 643.3Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.7 (77)
2 39.5 (119)
3 37.6 (121)
4 37.3 (76)
5 36.1 (140)
Top 5 longest reads and their mean basecall quality score
1 56487 (11.3)
2 54106 (11.0)
3 51114 (12.7)
4 39950 (11.0)
5 39056 (15.4)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot