NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 921.6
Mean read quality 14.3
Median read length 426.0
Median read quality 15.5
Number of reads 171,417.0
Read length N50 1,939.0
STDEV read length 1,437.7
Total bases 157,977,457.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 171410 (100.0%) 158.0Mb
>Q7 171391 (100.0%) 158.0Mb
>Q10 170412 (99.4%) 157.1Mb
>Q12 156235 (91.1%) 148.4Mb
>Q15 99115 (57.8%) 109.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.7 (39)
2 40.7 (29)
3 40.4 (98)
4 39.6 (16)
5 39.5 (146)
Top 5 longest reads and their mean basecall quality score
1 28117 (12.7)
2 27600 (17.8)
3 26167 (11.7)
4 24431 (15.5)
5 24101 (17.6)

Summary statistics after filtering

General summary
Mean read length 921.6
Mean read quality 14.3
Median read length 426.0
Median read quality 15.5
Number of reads 171,411.0
Read length N50 1,939.0
STDEV read length 1,437.7
Total bases 157,977,358.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 171404 (100.0%) 158.0Mb
>Q7 171385 (100.0%) 158.0Mb
>Q10 170406 (99.4%) 157.1Mb
>Q12 156229 (91.1%) 148.4Mb
>Q15 99109 (57.8%) 109.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.7 (39)
2 40.7 (29)
3 40.4 (98)
4 39.5 (146)
5 38.9 (57)
Top 5 longest reads and their mean basecall quality score
1 28117 (12.7)
2 27600 (17.8)
3 26167 (11.7)
4 24431 (15.5)
5 24101 (17.6)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot