NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,332.7
Mean read quality 14.7
Median read length 1,239.0
Median read quality 16.1
Number of reads 284,395.0
Read length N50 4,766.0
STDEV read length 2,829.3
Total bases 663,406,164.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 284392 (100.0%) 663.4Mb
>Q7 284372 (100.0%) 663.4Mb
>Q10 282705 (99.4%) 659.5Mb
>Q12 265422 (93.3%) 632.5Mb
>Q15 187748 (66.0%) 503.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.3 (18)
2 39.3 (10)
3 39.0 (27)
4 38.7 (39)
5 38.2 (102)
Top 5 longest reads and their mean basecall quality score
1 43375 (11.0)
2 34431 (14.8)
3 31646 (18.5)
4 31284 (15.6)
5 30736 (11.0)

Summary statistics after filtering

General summary
Mean read length 2,332.7
Mean read quality 14.7
Median read length 1,239.0
Median read quality 16.1
Number of reads 284,388.0
Read length N50 4,766.0
STDEV read length 2,829.3
Total bases 663,406,059.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 284385 (100.0%) 663.4Mb
>Q7 284365 (100.0%) 663.4Mb
>Q10 282698 (99.4%) 659.5Mb
>Q12 265415 (93.3%) 632.5Mb
>Q15 187741 (66.0%) 503.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.0 (27)
2 38.7 (39)
3 38.2 (102)
4 38.0 (57)
5 37.9 (175)
Top 5 longest reads and their mean basecall quality score
1 43375 (11.0)
2 34431 (14.8)
3 31646 (18.5)
4 31284 (15.6)
5 30736 (11.0)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot