NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 990.6
Mean read quality 13.7
Median read length 403.0
Median read quality 14.7
Number of reads 108,864.0
Read length N50 2,440.0
STDEV read length 1,562.8
Total bases 107,844,946.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 108849 (100.0%) 107.8Mb
>Q7 108815 (100.0%) 107.8Mb
>Q10 107594 (98.8%) 107.0Mb
>Q12 94416 (86.7%) 98.9Mb
>Q15 49839 (45.8%) 61.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.8 (15)
2 41.2 (16)
3 40.7 (16)
4 39.9 (16)
5 39.8 (16)
Top 5 longest reads and their mean basecall quality score
1 36406 (18.1)
2 32279 (17.5)
3 25296 (16.4)
4 24821 (11.8)
5 22782 (16.0)

Summary statistics after filtering

General summary
Mean read length 991.7
Mean read quality 13.6
Median read length 404.0
Median read quality 14.7
Number of reads 108,742.0
Read length N50 2,440.0
STDEV read length 1,563.4
Total bases 107,843,007.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 108727 (100.0%) 107.8Mb
>Q7 108693 (100.0%) 107.8Mb
>Q10 107472 (98.8%) 107.0Mb
>Q12 94294 (86.7%) 98.9Mb
>Q15 49717 (45.7%) 61.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.3 (131)
2 38.1 (28)
3 37.8 (32)
4 37.5 (25)
5 36.9 (27)
Top 5 longest reads and their mean basecall quality score
1 36406 (18.1)
2 32279 (17.5)
3 25296 (16.4)
4 24821 (11.8)
5 22782 (16.0)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot