NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,517.3
Mean read quality 13.9
Median read length 791.0
Median read quality 14.9
Number of reads 218,400.0
Read length N50 2,981.0
STDEV read length 1,978.5
Total bases 331,384,602.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 218398 (100.0%) 331.4Mb
>Q7 218386 (100.0%) 331.4Mb
>Q10 216970 (99.3%) 329.1Mb
>Q12 197151 (90.3%) 310.4Mb
>Q15 106875 (48.9%) 191.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.5 (16)
2 40.9 (52)
3 40.6 (112)
4 40.6 (10)
5 39.9 (34)
Top 5 longest reads and their mean basecall quality score
1 31776 (15.8)
2 28904 (14.9)
3 27865 (14.5)
4 27808 (15.8)
5 27274 (16.5)

Summary statistics after filtering

General summary
Mean read length 1,517.4
Mean read quality 13.9
Median read length 791.0
Median read quality 14.9
Number of reads 218,386.0
Read length N50 2,981.0
STDEV read length 1,978.5
Total bases 331,384,383.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 218384 (100.0%) 331.4Mb
>Q7 218372 (100.0%) 331.4Mb
>Q10 216956 (99.3%) 329.1Mb
>Q12 197137 (90.3%) 310.4Mb
>Q15 106861 (48.9%) 191.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.9 (52)
2 40.6 (112)
3 39.9 (34)
4 37.9 (94)
5 37.8 (60)
Top 5 longest reads and their mean basecall quality score
1 31776 (15.8)
2 28904 (14.9)
3 27865 (14.5)
4 27808 (15.8)
5 27274 (16.5)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot