NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 783.1
Mean read quality 13.6
Median read length 514.0
Median read quality 14.5
Number of reads 136,544.0
Read length N50 1,228.0
STDEV read length 825.4
Total bases 106,929,943.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 136534 (100.0%) 106.9Mb
>Q7 136491 (100.0%) 106.9Mb
>Q10 135166 (99.0%) 106.2Mb
>Q12 119417 (87.5%) 97.5Mb
>Q15 57487 (42.1%) 50.9Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.0 (21)
2 42.0 (21)
3 40.9 (16)
4 39.7 (10)
5 39.0 (16)
Top 5 longest reads and their mean basecall quality score
1 27020 (16.3)
2 22350 (16.9)
3 21251 (18.9)
4 19030 (16.6)
5 18931 (15.5)

Summary statistics after filtering

General summary
Mean read length 783.3
Mean read quality 13.6
Median read length 514.0
Median read quality 14.5
Number of reads 136,511.0
Read length N50 1,228.0
STDEV read length 825.4
Total bases 106,929,432.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 136501 (100.0%) 106.9Mb
>Q7 136458 (100.0%) 106.9Mb
>Q10 135133 (99.0%) 106.2Mb
>Q12 119384 (87.5%) 97.5Mb
>Q15 57454 (42.1%) 50.9Mb
Top 5 highest mean basecall quality scores and their read lengths
1 43.0 (21)
2 42.0 (21)
3 38.0 (21)
4 38.0 (21)
5 37.4 (21)
Top 5 longest reads and their mean basecall quality score
1 27020 (16.3)
2 22350 (16.9)
3 21251 (18.9)
4 19030 (16.6)
5 18931 (15.5)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot