NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,094.4
Mean read quality 14.2
Median read length 946.0
Median read quality 15.5
Number of reads 194,550.0
Read length N50 4,741.0
STDEV read length 2,805.2
Total bases 407,467,498.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 194545 (100.0%) 407.5Mb
>Q7 194528 (100.0%) 407.5Mb
>Q10 192734 (99.1%) 404.6Mb
>Q12 175632 (90.3%) 384.1Mb
>Q15 112612 (57.9%) 289.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 44.6 (11)
2 40.1 (10)
3 37.7 (13)
4 37.6 (24)
5 37.5 (314)
Top 5 longest reads and their mean basecall quality score
1 35151 (11.1)
2 32946 (11.8)
3 32007 (14.0)
4 30840 (16.2)
5 30264 (16.2)

Summary statistics after filtering

General summary
Mean read length 2,094.5
Mean read quality 14.2
Median read length 946.0
Median read quality 15.5
Number of reads 194,545.0
Read length N50 4,741.0
STDEV read length 2,805.3
Total bases 407,467,443.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 194540 (100.0%) 407.5Mb
>Q7 194523 (100.0%) 407.5Mb
>Q10 192729 (99.1%) 404.6Mb
>Q12 175627 (90.3%) 384.1Mb
>Q15 112607 (57.9%) 289.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 37.6 (24)
2 37.5 (314)
3 36.4 (155)
4 36.0 (98)
5 35.5 (117)
Top 5 longest reads and their mean basecall quality score
1 35151 (11.1)
2 32946 (11.8)
3 32007 (14.0)
4 30840 (16.2)
5 30264 (16.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot