NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,943.3
Mean read quality 14.5
Median read length 1,056.0
Median read quality 15.8
Number of reads 260,739.0
Read length N50 3,846.0
STDEV read length 2,430.2
Total bases 506,705,720.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 260730 (100.0%) 506.7Mb
>Q7 260693 (100.0%) 506.7Mb
>Q10 258910 (99.3%) 503.7Mb
>Q12 241331 (92.6%) 482.2Mb
>Q15 162849 (62.5%) 368.9Mb
Top 5 highest mean basecall quality scores and their read lengths
1 44.0 (16)
2 40.3 (16)
3 40.2 (26)
4 39.9 (16)
5 39.5 (26)
Top 5 longest reads and their mean basecall quality score
1 37899 (12.1)
2 35600 (12.1)
3 33487 (13.4)
4 33325 (10.9)
5 31937 (12.7)

Summary statistics after filtering

General summary
Mean read length 1,943.5
Mean read quality 14.5
Median read length 1,056.0
Median read quality 15.8
Number of reads 260,716.0
Read length N50 3,846.0
STDEV read length 2,430.3
Total bases 506,705,358.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 260707 (100.0%) 506.7Mb
>Q7 260670 (100.0%) 506.7Mb
>Q10 258887 (99.3%) 503.7Mb
>Q12 241308 (92.6%) 482.2Mb
>Q15 162826 (62.5%) 368.9Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.2 (26)
2 39.5 (26)
3 39.3 (26)
4 39.0 (93)
5 38.6 (26)
Top 5 longest reads and their mean basecall quality score
1 37899 (12.1)
2 35600 (12.1)
3 33487 (13.4)
4 33325 (10.9)
5 31937 (12.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot