NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,865.2
Mean read quality 15.2
Median read length 922.0
Median read quality 16.7
Number of reads 289,795.0
Read length N50 3,903.0
STDEV read length 2,533.1
Total bases 540,518,203.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 289776 (100.0%) 540.5Mb
>Q7 289697 (100.0%) 540.5Mb
>Q10 287906 (99.3%) 537.7Mb
>Q12 272842 (94.2%) 518.1Mb
>Q15 209742 (72.4%) 433.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.3 (16)
2 42.1 (16)
3 42.0 (16)
4 41.9 (16)
5 41.8 (16)
Top 5 longest reads and their mean basecall quality score
1 70276 (12.5)
2 56296 (12.7)
3 50589 (17.3)
4 47698 (11.3)
5 45671 (11.7)

Summary statistics after filtering

General summary
Mean read length 1,866.6
Mean read quality 15.2
Median read length 923.0
Median read quality 16.7
Number of reads 289,578.0
Read length N50 3,903.0
STDEV read length 2,533.5
Total bases 540,514,768.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 289559 (100.0%) 540.5Mb
>Q7 289480 (100.0%) 540.5Mb
>Q10 287689 (99.3%) 537.7Mb
>Q12 272625 (94.1%) 518.1Mb
>Q15 209525 (72.4%) 433.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.2 (175)
2 38.8 (196)
3 38.1 (223)
4 37.8 (194)
5 37.7 (178)
Top 5 longest reads and their mean basecall quality score
1 70276 (12.5)
2 56296 (12.7)
3 50589 (17.3)
4 47698 (11.3)
5 45671 (11.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot