NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,358.4
Mean read quality 15.1
Median read length 1,420.0
Median read quality 16.5
Number of reads 614,481.0
Read length N50 4,274.0
STDEV read length 2,690.1
Total bases 1,449,215,027.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 614468 (100.0%) 1449.2Mb
>Q7 614437 (100.0%) 1449.2Mb
>Q10 610870 (99.4%) 1439.9Mb
>Q12 580055 (94.4%) 1379.7Mb
>Q15 443587 (72.2%) 1125.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.6 (18)
2 41.5 (18)
3 41.4 (15)
4 41.0 (18)
5 40.9 (17)
Top 5 longest reads and their mean basecall quality score
1 52850 (11.2)
2 51838 (11.4)
3 48765 (11.2)
4 45849 (10.9)
5 38418 (11.6)

Summary statistics after filtering

General summary
Mean read length 2,358.6
Mean read quality 15.1
Median read length 1,420.0
Median read quality 16.5
Number of reads 614,446.0
Read length N50 4,274.0
STDEV read length 2,690.1
Total bases 1,449,214,465.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 614433 (100.0%) 1449.2Mb
>Q7 614402 (100.0%) 1449.2Mb
>Q10 610835 (99.4%) 1439.9Mb
>Q12 580020 (94.4%) 1379.7Mb
>Q15 443552 (72.2%) 1125.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.4 (72)
2 38.4 (58)
3 38.1 (209)
4 38.0 (135)
5 37.9 (104)
Top 5 longest reads and their mean basecall quality score
1 52850 (11.2)
2 51838 (11.4)
3 48765 (11.2)
4 45849 (10.9)
5 38418 (11.6)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot