NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 889.4
Mean read quality 14.2
Median read length 500.0
Median read quality 15.4
Number of reads 134,407.0
Read length N50 1,588.0
STDEV read length 1,079.7
Total bases 119,541,391.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 134392 (100.0%) 119.5Mb
>Q7 134342 (100.0%) 119.5Mb
>Q10 133207 (99.1%) 118.8Mb
>Q12 121285 (90.2%) 111.9Mb
>Q15 75541 (56.2%) 79.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.4 (26)
2 42.3 (26)
3 41.1 (26)
4 41.1 (45)
5 41.1 (46)
Top 5 longest reads and their mean basecall quality score
1 22059 (17.5)
2 21324 (17.0)
3 19900 (15.5)
4 19040 (12.4)
5 18175 (17.4)

Summary statistics after filtering

General summary
Mean read length 889.6
Mean read quality 14.2
Median read length 500.0
Median read quality 15.4
Number of reads 134,377.0
Read length N50 1,588.0
STDEV read length 1,079.7
Total bases 119,540,964.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 134362 (100.0%) 119.5Mb
>Q7 134312 (100.0%) 119.5Mb
>Q10 133177 (99.1%) 118.8Mb
>Q12 121255 (90.2%) 111.9Mb
>Q15 75511 (56.2%) 79.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.4 (26)
2 42.3 (26)
3 41.1 (26)
4 41.1 (45)
5 41.1 (46)
Top 5 longest reads and their mean basecall quality score
1 22059 (17.5)
2 21324 (17.0)
3 19900 (15.5)
4 19040 (12.4)
5 18175 (17.4)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot