NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,162.7
Mean read quality 14.4
Median read length 693.0
Median read quality 15.6
Number of reads 558,338.0
Read length N50 2,043.0
STDEV read length 1,317.1
Total bases 649,154,333.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 558331 (100.0%) 649.2Mb
>Q7 558298 (100.0%) 649.2Mb
>Q10 554564 (99.3%) 645.2Mb
>Q12 512024 (91.7%) 612.9Mb
>Q15 332563 (59.6%) 450.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.6 (10)
2 39.9 (156)
3 39.6 (10)
4 39.6 (133)
5 39.5 (76)
Top 5 longest reads and their mean basecall quality score
1 43832 (19.7)
2 26244 (18.3)
3 25908 (12.0)
4 23626 (13.3)
5 22347 (17.7)

Summary statistics after filtering

General summary
Mean read length 1,162.7
Mean read quality 14.4
Median read length 693.0
Median read quality 15.6
Number of reads 558,325.0
Read length N50 2,043.0
STDEV read length 1,317.1
Total bases 649,154,166.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 558318 (100.0%) 649.2Mb
>Q7 558285 (100.0%) 649.2Mb
>Q10 554551 (99.3%) 645.2Mb
>Q12 512011 (91.7%) 612.9Mb
>Q15 332550 (59.6%) 450.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 39.9 (156)
2 39.6 (133)
3 39.5 (76)
4 39.0 (172)
5 38.7 (42)
Top 5 longest reads and their mean basecall quality score
1 43832 (19.7)
2 26244 (18.3)
3 25908 (12.0)
4 23626 (13.3)
5 22347 (17.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot