NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 635.1
Mean read quality 14.2
Median read length 404.0
Median read quality 15.3
Number of reads 149,745.0
Read length N50 987.0
STDEV read length 684.3
Total bases 95,109,837.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 149734 (100.0%) 95.1Mb
>Q7 149708 (100.0%) 95.1Mb
>Q10 148753 (99.3%) 94.6Mb
>Q12 136283 (91.0%) 88.8Mb
>Q15 82282 (54.9%) 58.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 44.4 (16)
2 42.6 (16)
3 42.5 (38)
4 42.2 (16)
5 41.9 (16)
Top 5 longest reads and their mean basecall quality score
1 17314 (17.2)
2 16876 (17.5)
3 16021 (18.1)
4 13946 (16.8)
5 12994 (17.7)

Summary statistics after filtering

General summary
Mean read length 635.3
Mean read quality 14.2
Median read length 404.0
Median read quality 15.3
Number of reads 149,696.0
Read length N50 987.0
STDEV read length 684.4
Total bases 95,109,062.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 149685 (100.0%) 95.1Mb
>Q7 149659 (100.0%) 95.1Mb
>Q10 148704 (99.3%) 94.6Mb
>Q12 136234 (91.0%) 88.8Mb
>Q15 82233 (54.9%) 58.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 42.5 (38)
2 41.1 (30)
3 40.1 (41)
4 40.0 (109)
5 40.0 (140)
Top 5 longest reads and their mean basecall quality score
1 17314 (17.2)
2 16876 (17.5)
3 16021 (18.1)
4 13946 (16.8)
5 12994 (17.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot