NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,440.4
Mean read quality 13.7
Median read length 787.0
Median read quality 14.8
Number of reads 144,084.0
Read length N50 2,674.0
STDEV read length 1,876.2
Total bases 207,541,193.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 144076 (100.0%) 207.5Mb
>Q7 144054 (100.0%) 207.5Mb
>Q10 142873 (99.2%) 205.8Mb
>Q12 128538 (89.2%) 192.8Mb
>Q15 66498 (46.2%) 113.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 40.2 (10)
2 38.2 (10)
3 38.0 (103)
4 37.2 (10)
5 37.0 (20)
Top 5 longest reads and their mean basecall quality score
1 36852 (13.5)
2 34635 (15.1)
3 31845 (17.2)
4 30909 (12.7)
5 30392 (14.7)

Summary statistics after filtering

General summary
Mean read length 1,440.5
Mean read quality 13.7
Median read length 787.0
Median read quality 14.8
Number of reads 144,077.0
Read length N50 2,674.0
STDEV read length 1,876.2
Total bases 207,541,123.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 144069 (100.0%) 207.5Mb
>Q7 144047 (100.0%) 207.5Mb
>Q10 142866 (99.2%) 205.8Mb
>Q12 128531 (89.2%) 192.8Mb
>Q15 66491 (46.1%) 113.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 38.0 (103)
2 37.0 (20)
3 34.0 (128)
4 33.8 (207)
5 33.8 (156)
Top 5 longest reads and their mean basecall quality score
1 36852 (13.5)
2 34635 (15.1)
3 31845 (17.2)
4 30909 (12.7)
5 30392 (14.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot