NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,716.7
Mean read quality 13.8
Median read length 764.0
Median read quality 14.9
Number of reads 174,244.0
Read length N50 3,860.0
STDEV read length 2,418.5
Total bases 299,129,269.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 174236 (100.0%) 299.1Mb
>Q7 174210 (100.0%) 299.1Mb
>Q10 172620 (99.1%) 296.8Mb
>Q12 154769 (88.8%) 279.2Mb
>Q15 82839 (47.5%) 172.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.5 (23)
2 40.2 (23)
3 39.2 (160)
4 37.4 (23)
5 37.3 (96)
Top 5 longest reads and their mean basecall quality score
1 39120 (12.8)
2 38223 (14.0)
3 35277 (16.6)
4 33693 (16.2)
5 32153 (16.5)

Summary statistics after filtering

General summary
Mean read length 1,716.8
Mean read quality 13.8
Median read length 764.0
Median read quality 14.9
Number of reads 174,240.0
Read length N50 3,860.0
STDEV read length 2,418.5
Total bases 299,129,222.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 174232 (100.0%) 299.1Mb
>Q7 174206 (100.0%) 299.1Mb
>Q10 172616 (99.1%) 296.8Mb
>Q12 154765 (88.8%) 279.2Mb
>Q15 82835 (47.5%) 172.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 41.5 (23)
2 40.2 (23)
3 39.2 (160)
4 37.4 (23)
5 37.3 (96)
Top 5 longest reads and their mean basecall quality score
1 39120 (12.8)
2 38223 (14.0)
3 35277 (16.6)
4 33693 (16.2)
5 32153 (16.5)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot