NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 694.1
Mean read quality 9.8
Median read length 248.0
Median read quality 10.5
Number of reads 62,115.0
Read length N50 1,940.0
STDEV read length 943.3
Total bases 43,113,194.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 61888 (99.6%) 43.1Mb
>Q7 60647 (97.6%) 43.0Mb
>Q10 40134 (64.6%) 25.9Mb
>Q12 13368 (21.5%) 1.3Mb
>Q15 4022 (6.5%) 0.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.8 (17)
2 31.6 (16)
3 30.9 (18)
4 30.4 (16)
5 29.8 (20)
Top 5 longest reads and their mean basecall quality score
1 7342 (8.0)
2 6984 (8.1)
3 6666 (9.0)
4 5840 (10.1)
5 5702 (8.5)

Summary statistics after filtering

General summary
Mean read length 694.1
Mean read quality 9.8
Median read length 248.0
Median read quality 10.5
Number of reads 62,111.0
Read length N50 1,940.0
STDEV read length 943.4
Total bases 43,113,127.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 61884 (99.6%) 43.1Mb
>Q7 60643 (97.6%) 43.0Mb
>Q10 40130 (64.6%) 25.9Mb
>Q12 13364 (21.5%) 1.3Mb
>Q15 4018 (6.5%) 0.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.8 (20)
2 28.4 (166)
3 28.1 (36)
4 28.0 (16)
5 27.5 (21)
Top 5 longest reads and their mean basecall quality score
1 7342 (8.0)
2 6984 (8.1)
3 6666 (9.0)
4 5840 (10.1)
5 5702 (8.5)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot