NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 2,215.6
Mean read quality 9.9
Median read length 2,147.0
Median read quality 10.6
Number of reads 80,967.0
Read length N50 2,861.0
STDEV read length 2,514.8
Total bases 179,393,827.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 80921 (99.9%) 179.4Mb
>Q7 80633 (99.6%) 179.4Mb
>Q10 59282 (73.2%) 135.9Mb
>Q12 8402 (10.4%) 14.6Mb
>Q15 1628 (2.0%) 0.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.0 (16)
2 29.3 (16)
3 28.9 (17)
4 28.6 (45)
5 28.4 (16)
Top 5 longest reads and their mean basecall quality score
1 81388 (12.5)
2 79394 (13.5)
3 77907 (13.0)
4 74363 (13.8)
5 74033 (9.7)

Summary statistics after filtering

General summary
Mean read length 2,215.7
Mean read quality 9.9
Median read length 2,147.0
Median read quality 10.6
Number of reads 80,966.0
Read length N50 2,861.0
STDEV read length 2,514.8
Total bases 179,393,811.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 80920 (99.9%) 179.4Mb
>Q7 80632 (99.6%) 179.4Mb
>Q10 59281 (73.2%) 135.9Mb
>Q12 8401 (10.4%) 14.6Mb
>Q15 1627 (2.0%) 0.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.3 (16)
2 28.9 (17)
3 28.6 (45)
4 28.4 (16)
5 28.0 (17)
Top 5 longest reads and their mean basecall quality score
1 81388 (12.5)
2 79394 (13.5)
3 77907 (13.0)
4 74363 (13.8)
5 74033 (9.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot