NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 942.6
Mean read quality 9.3
Median read length 317.0
Median read quality 10.0
Number of reads 34,871.0
Read length N50 2,233.0
STDEV read length 1,086.8
Total bases 32,869,674.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 34702 (99.5%) 32.9Mb
>Q7 33743 (96.8%) 32.8Mb
>Q10 17366 (49.8%) 13.6Mb
>Q12 5704 (16.4%) 0.6Mb
>Q15 1480 (4.2%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.5 (18)
2 28.8 (18)
3 28.5 (17)
4 28.3 (20)
5 27.8 (23)
Top 5 longest reads and their mean basecall quality score
1 7794 (7.6)
2 7550 (9.7)
3 6931 (8.0)
4 6651 (8.5)
5 6475 (8.7)

Summary statistics after filtering

General summary
Mean read length 942.6
Mean read quality 9.3
Median read length 317.0
Median read quality 10.0
Number of reads 34,870.0
Read length N50 2,233.0
STDEV read length 1,086.8
Total bases 32,869,656.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 34701 (99.5%) 32.9Mb
>Q7 33742 (96.8%) 32.8Mb
>Q10 17365 (49.8%) 13.6Mb
>Q12 5703 (16.4%) 0.6Mb
>Q15 1479 (4.2%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 28.8 (18)
2 28.5 (17)
3 28.3 (20)
4 27.8 (23)
5 27.2 (17)
Top 5 longest reads and their mean basecall quality score
1 7794 (7.6)
2 7550 (9.7)
3 6931 (8.0)
4 6651 (8.5)
5 6475 (8.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot