NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 497.3
Mean read quality 10.1
Median read length 366.0
Median read quality 11.2
Number of reads 40,427.0
Read length N50 676.0
STDEV read length 406.7
Total bases 20,103,107.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 40411 (100.0%) 20.1Mb
>Q7 40093 (99.2%) 20.1Mb
>Q10 25310 (62.6%) 13.7Mb
>Q12 17190 (42.5%) 9.6Mb
>Q15 6598 (16.3%) 3.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.9 (10)
2 32.4 (19)
3 32.2 (20)
4 32.1 (20)
5 31.9 (20)
Top 5 longest reads and their mean basecall quality score
1 3270 (13.5)
2 3262 (10.0)
3 3124 (12.0)
4 2980 (14.1)
5 2976 (13.2)

Summary statistics after filtering

General summary
Mean read length 497.3
Mean read quality 10.1
Median read length 366.0
Median read quality 11.2
Number of reads 40,423.0
Read length N50 676.0
STDEV read length 406.6
Total bases 20,103,055.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 40407 (100.0%) 20.1Mb
>Q7 40089 (99.2%) 20.1Mb
>Q10 25306 (62.6%) 13.7Mb
>Q12 17186 (42.5%) 9.6Mb
>Q15 6594 (16.3%) 3.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.2 (20)
2 32.1 (20)
3 31.9 (20)
4 31.8 (20)
5 31.7 (20)
Top 5 longest reads and their mean basecall quality score
1 3270 (13.5)
2 3262 (10.0)
3 3124 (12.0)
4 2980 (14.1)
5 2976 (13.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot