NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 519.5
Mean read quality 10.0
Median read length 387.0
Median read quality 10.9
Number of reads 20,413.0
Read length N50 720.0
STDEV read length 422.3
Total bases 10,603,626.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 20398 (99.9%) 10.6Mb
>Q7 20227 (99.1%) 10.6Mb
>Q10 12052 (59.0%) 7.0Mb
>Q12 8179 (40.1%) 4.9Mb
>Q15 3223 (15.8%) 1.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.8 (20)
2 32.9 (20)
3 32.6 (20)
4 32.6 (20)
5 31.6 (20)
Top 5 longest reads and their mean basecall quality score
1 4639 (7.4)
2 3628 (8.9)
3 3437 (13.8)
4 3248 (12.4)
5 3199 (14.3)

Summary statistics after filtering

General summary
Mean read length 519.5
Mean read quality 10.0
Median read length 387.0
Median read quality 10.9
Number of reads 20,412.0
Read length N50 720.0
STDEV read length 422.3
Total bases 10,603,607.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 20397 (99.9%) 10.6Mb
>Q7 20226 (99.1%) 10.6Mb
>Q10 12051 (59.0%) 7.0Mb
>Q12 8178 (40.1%) 4.9Mb
>Q15 3222 (15.8%) 1.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.8 (20)
2 32.9 (20)
3 32.6 (20)
4 32.6 (20)
5 31.6 (20)
Top 5 longest reads and their mean basecall quality score
1 4639 (7.4)
2 3628 (8.9)
3 3437 (13.8)
4 3248 (12.4)
5 3199 (14.3)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot