NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,259.1
Mean read quality 12.5
Median read length 1,186.0
Median read quality 14.0
Number of reads 386,409.0
Read length N50 1,399.0
STDEV read length 505.1
Total bases 486,533,338.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 386402 (100.0%) 486.5Mb
>Q7 386350 (100.0%) 486.5Mb
>Q10 355365 (92.0%) 448.8Mb
>Q12 300867 (77.9%) 381.0Mb
>Q15 118412 (30.6%) 146.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.2 (16)
2 32.1 (20)
3 30.9 (20)
4 30.4 (20)
5 30.0 (20)
Top 5 longest reads and their mean basecall quality score
1 8928 (11.3)
2 6763 (14.4)
3 6021 (7.9)
4 4630 (8.1)
5 4443 (10.6)

Summary statistics after filtering

General summary
Mean read length 1,259.1
Mean read quality 12.5
Median read length 1,186.0
Median read quality 14.0
Number of reads 386,408.0
Read length N50 1,399.0
STDEV read length 505.1
Total bases 486,533,322.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 386401 (100.0%) 486.5Mb
>Q7 386349 (100.0%) 486.5Mb
>Q10 355364 (92.0%) 448.8Mb
>Q12 300866 (77.9%) 381.0Mb
>Q15 118411 (30.6%) 146.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.1 (20)
2 30.9 (20)
3 30.4 (20)
4 30.0 (20)
5 29.6 (20)
Top 5 longest reads and their mean basecall quality score
1 8928 (11.3)
2 6763 (14.4)
3 6021 (7.9)
4 4630 (8.1)
5 4443 (10.6)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot