NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 534.5
Mean read quality 10.0
Median read length 336.0
Median read quality 10.9
Number of reads 8,321.0
Read length N50 917.0
STDEV read length 523.0
Total bases 4,447,401.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 8319 (100.0%) 4.4Mb
>Q7 8261 (99.3%) 4.4Mb
>Q10 4964 (59.7%) 2.8Mb
>Q12 3197 (38.4%) 1.9Mb
>Q15 953 (11.5%) 0.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.9 (15)
2 30.4 (20)
3 30.0 (20)
4 28.9 (20)
5 28.9 (20)
Top 5 longest reads and their mean basecall quality score
1 3343 (14.1)
2 3322 (14.1)
3 3231 (12.2)
4 3192 (10.0)
5 3175 (11.2)

Summary statistics after filtering

General summary
Mean read length 534.5
Mean read quality 10.0
Median read length 336.0
Median read quality 10.9
Number of reads 8,320.0
Read length N50 917.0
STDEV read length 523.0
Total bases 4,447,386.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 8318 (100.0%) 4.4Mb
>Q7 8260 (99.3%) 4.4Mb
>Q10 4963 (59.7%) 2.8Mb
>Q12 3196 (38.4%) 1.9Mb
>Q15 952 (11.4%) 0.5Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.4 (20)
2 30.0 (20)
3 28.9 (20)
4 28.9 (20)
5 28.9 (21)
Top 5 longest reads and their mean basecall quality score
1 3343 (14.1)
2 3322 (14.1)
3 3231 (12.2)
4 3192 (10.0)
5 3175 (11.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot