NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 899.5
Mean read quality 12.3
Median read length 839.0
Median read quality 13.6
Number of reads 291,635.0
Read length N50 1,036.0
STDEV read length 419.6
Total bases 262,331,071.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 291610 (100.0%) 262.3Mb
>Q7 291260 (99.9%) 262.3Mb
>Q10 265062 (90.9%) 240.4Mb
>Q12 217106 (74.4%) 197.8Mb
>Q15 75482 (25.9%) 65.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.3 (20)
2 32.1 (20)
3 32.1 (19)
4 31.9 (20)
5 31.7 (20)
Top 5 longest reads and their mean basecall quality score
1 4949 (10.9)
2 3869 (12.4)
3 3852 (14.2)
4 3771 (8.5)
5 3400 (12.7)

Summary statistics after filtering

General summary
Mean read length 899.5
Mean read quality 12.3
Median read length 839.0
Median read quality 13.6
Number of reads 291,629.0
Read length N50 1,036.0
STDEV read length 419.6
Total bases 262,330,957.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 291604 (100.0%) 262.3Mb
>Q7 291254 (99.9%) 262.3Mb
>Q10 265056 (90.9%) 240.4Mb
>Q12 217100 (74.4%) 197.8Mb
>Q15 75476 (25.9%) 65.7Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.3 (20)
2 32.1 (20)
3 31.9 (20)
4 31.7 (20)
5 31.7 (20)
Top 5 longest reads and their mean basecall quality score
1 4949 (10.9)
2 3869 (12.4)
3 3852 (14.2)
4 3771 (8.5)
5 3400 (12.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot