NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 904.9
Mean read quality 12.4
Median read length 844.0
Median read quality 13.8
Number of reads 295,313.0
Read length N50 1,035.0
STDEV read length 416.5
Total bases 267,237,278.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 295298 (100.0%) 267.2Mb
>Q7 294977 (99.9%) 267.2Mb
>Q10 269690 (91.3%) 245.7Mb
>Q12 223843 (75.8%) 204.6Mb
>Q15 83351 (28.2%) 73.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.3 (17)
2 33.1 (18)
3 32.9 (20)
4 32.1 (20)
5 32.1 (21)
Top 5 longest reads and their mean basecall quality score
1 17531 (8.0)
2 4345 (7.8)
3 3804 (8.2)
4 3577 (8.3)
5 3503 (11.2)

Summary statistics after filtering

General summary
Mean read length 904.9
Mean read quality 12.4
Median read length 844.0
Median read quality 13.8
Number of reads 295,307.0
Read length N50 1,035.0
STDEV read length 416.5
Total bases 267,237,169.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 295292 (100.0%) 267.2Mb
>Q7 294971 (99.9%) 267.2Mb
>Q10 269684 (91.3%) 245.7Mb
>Q12 223837 (75.8%) 204.6Mb
>Q15 83345 (28.2%) 73.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.9 (20)
2 32.1 (20)
3 32.1 (21)
4 31.9 (20)
5 31.9 (20)
Top 5 longest reads and their mean basecall quality score
1 17531 (8.0)
2 4345 (7.8)
3 3804 (8.2)
4 3577 (8.3)
5 3503 (11.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot