NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,279.8
Mean read quality 11.8
Median read length 1,262.0
Median read quality 13.1
Number of reads 199,117.0
Read length N50 1,499.0
STDEV read length 550.4
Total bases 254,836,190.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 199091 (100.0%) 254.8Mb
>Q7 198964 (99.9%) 254.8Mb
>Q10 176985 (88.9%) 228.8Mb
>Q12 136955 (68.8%) 177.9Mb
>Q15 24839 (12.5%) 29.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.1 (20)
2 31.6 (20)
3 30.7 (20)
4 30.6 (21)
5 30.1 (12)
Top 5 longest reads and their mean basecall quality score
1 4381 (10.9)
2 4159 (13.5)
3 4145 (10.4)
4 3828 (12.6)
5 3817 (11.2)

Summary statistics after filtering

General summary
Mean read length 1,279.8
Mean read quality 11.8
Median read length 1,262.0
Median read quality 13.1
Number of reads 199,116.0
Read length N50 1,499.0
STDEV read length 550.4
Total bases 254,836,178.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 199090 (100.0%) 254.8Mb
>Q7 198963 (99.9%) 254.8Mb
>Q10 176984 (88.9%) 228.8Mb
>Q12 136954 (68.8%) 177.9Mb
>Q15 24838 (12.5%) 29.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.1 (20)
2 31.6 (20)
3 30.7 (20)
4 30.6 (21)
5 30.0 (20)
Top 5 longest reads and their mean basecall quality score
1 4381 (10.9)
2 4159 (13.5)
3 4145 (10.4)
4 3828 (12.6)
5 3817 (11.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot