NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 311.0
Mean read quality 9.6
Median read length 293.0
Median read quality 10.3
Number of reads 78,985.0
Read length N50 363.0
STDEV read length 173.7
Total bases 24,565,555.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 78980 (100.0%) 24.6Mb
>Q7 78621 (99.5%) 24.5Mb
>Q10 42215 (53.4%) 12.0Mb
>Q12 25653 (32.5%) 7.2Mb
>Q15 9168 (11.6%) 2.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 34.8 (10)
2 32.1 (20)
3 30.8 (19)
4 30.8 (36)
5 30.3 (20)
Top 5 longest reads and their mean basecall quality score
1 14706 (7.6)
2 3206 (8.1)
3 2825 (12.8)
4 2753 (12.6)
5 2668 (8.9)

Summary statistics after filtering

General summary
Mean read length 311.0
Mean read quality 9.6
Median read length 293.0
Median read quality 10.3
Number of reads 78,983.0
Read length N50 363.0
STDEV read length 173.7
Total bases 24,565,526.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 78978 (100.0%) 24.6Mb
>Q7 78619 (99.5%) 24.5Mb
>Q10 42213 (53.4%) 12.0Mb
>Q12 25651 (32.5%) 7.2Mb
>Q15 9166 (11.6%) 2.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 32.1 (20)
2 30.8 (36)
3 30.3 (20)
4 30.0 (14)
5 29.8 (38)
Top 5 longest reads and their mean basecall quality score
1 14706 (7.6)
2 3206 (8.1)
3 2825 (12.8)
4 2753 (12.6)
5 2668 (8.9)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot