NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 330.4
Mean read quality 9.7
Median read length 300.0
Median read quality 10.3
Number of reads 112,189.0
Read length N50 391.0
STDEV read length 199.0
Total bases 37,071,904.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 112182 (100.0%) 37.1Mb
>Q7 111590 (99.5%) 37.0Mb
>Q10 60067 (53.5%) 18.3Mb
>Q12 38914 (34.7%) 11.8Mb
>Q15 16231 (14.5%) 4.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 34.0 (64)
2 33.9 (16)
3 33.2 (14)
4 32.6 (13)
5 32.5 (20)
Top 5 longest reads and their mean basecall quality score
1 20688 (8.5)
2 7748 (7.7)
3 5084 (7.9)
4 3260 (8.7)
5 2710 (12.8)

Summary statistics after filtering

General summary
Mean read length 330.5
Mean read quality 9.7
Median read length 300.0
Median read quality 10.3
Number of reads 112,184.0
Read length N50 391.0
STDEV read length 198.9
Total bases 37,071,823.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 112177 (100.0%) 37.1Mb
>Q7 111585 (99.5%) 37.0Mb
>Q10 60062 (53.5%) 18.3Mb
>Q12 38909 (34.7%) 11.8Mb
>Q15 16226 (14.5%) 4.8Mb
Top 5 highest mean basecall quality scores and their read lengths
1 34.0 (64)
2 32.5 (20)
3 32.4 (20)
4 32.2 (51)
5 32.0 (21)
Top 5 longest reads and their mean basecall quality score
1 20688 (8.5)
2 7748 (7.7)
3 5084 (7.9)
4 3260 (8.7)
5 2710 (12.8)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot