NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 4,213.2
Mean read quality 11.9
Median read length 2,372.0
Median read quality 12.9
Number of reads 91,536.0
Read length N50 8,215.0
STDEV read length 5,187.2
Total bases 385,660,532.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 91535 (100.0%) 385.7Mb
>Q7 91524 (100.0%) 385.7Mb
>Q10 85585 (93.5%) 359.8Mb
>Q12 60491 (66.1%) 250.7Mb
>Q15 11122 (12.2%) 32.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.1 (10)
2 28.9 (10)
3 26.2 (122)
4 26.0 (10)
5 23.4 (160)
Top 5 longest reads and their mean basecall quality score
1 79503 (11.7)
2 69673 (13.1)
3 68258 (12.6)
4 62662 (10.2)
5 62065 (10.8)

Summary statistics after filtering

General summary
Mean read length 4,213.3
Mean read quality 11.9
Median read length 2,372.0
Median read quality 12.9
Number of reads 91,535.0
Read length N50 8,215.0
STDEV read length 5,187.2
Total bases 385,660,522.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 91534 (100.0%) 385.7Mb
>Q7 91523 (100.0%) 385.7Mb
>Q10 85584 (93.5%) 359.8Mb
>Q12 60490 (66.1%) 250.7Mb
>Q15 11121 (12.1%) 32.2Mb
Top 5 highest mean basecall quality scores and their read lengths
1 28.9 (10)
2 26.2 (122)
3 26.0 (10)
4 23.4 (160)
5 23.3 (32)
Top 5 longest reads and their mean basecall quality score
1 79503 (11.7)
2 69673 (13.1)
3 68258 (12.6)
4 62662 (10.2)
5 62065 (10.8)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot