NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 3,965.7
Mean read quality 12.6
Median read length 2,531.0
Median read quality 13.7
Number of reads 137,229.0
Read length N50 7,105.0
STDEV read length 4,405.0
Total bases 544,211,048.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 137223 (100.0%) 544.2Mb
>Q7 137124 (99.9%) 544.2Mb
>Q10 130749 (95.3%) 520.9Mb
>Q12 104564 (76.2%) 417.5Mb
>Q15 38154 (27.8%) 139.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.3 (10)
2 29.7 (10)
3 27.6 (10)
4 26.9 (10)
5 26.8 (10)
Top 5 longest reads and their mean basecall quality score
1 65220 (11.6)
2 62649 (17.0)
3 61658 (15.1)
4 57332 (11.9)
5 56920 (13.8)

Summary statistics after filtering

General summary
Mean read length 3,965.7
Mean read quality 12.6
Median read length 2,531.5
Median read quality 13.7
Number of reads 137,228.0
Read length N50 7,105.0
STDEV read length 4,405.0
Total bases 544,211,038.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 137222 (100.0%) 544.2Mb
>Q7 137123 (99.9%) 544.2Mb
>Q10 130748 (95.3%) 520.9Mb
>Q12 104563 (76.2%) 417.5Mb
>Q15 38153 (27.8%) 139.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.7 (10)
2 27.6 (10)
3 26.9 (10)
4 26.8 (10)
5 26.6 (10)
Top 5 longest reads and their mean basecall quality score
1 65220 (11.6)
2 62649 (17.0)
3 61658 (15.1)
4 57332 (11.9)
5 56920 (13.8)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot