NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 3,845.8
Mean read quality 12.7
Median read length 2,234.0
Median read quality 13.9
Number of reads 233,996.0
Read length N50 7,037.0
STDEV read length 4,995.3
Total bases 899,900,396.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 233986 (100.0%) 899.9Mb
>Q7 233892 (100.0%) 899.9Mb
>Q10 223803 (95.6%) 861.1Mb
>Q12 181346 (77.5%) 700.4Mb
>Q15 68952 (29.5%) 247.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.2 (10)
2 29.6 (10)
3 29.3 (10)
4 28.5 (10)
5 28.4 (10)
Top 5 longest reads and their mean basecall quality score
1 98675 (14.2)
2 97915 (13.3)
3 87255 (12.2)
4 87040 (14.8)
5 83657 (15.2)

Summary statistics after filtering

General summary
Mean read length 3,845.8
Mean read quality 12.7
Median read length 2,234.0
Median read quality 13.9
Number of reads 233,995.0
Read length N50 7,037.0
STDEV read length 4,995.3
Total bases 899,900,386.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 233985 (100.0%) 899.9Mb
>Q7 233891 (100.0%) 899.9Mb
>Q10 223802 (95.6%) 861.1Mb
>Q12 181345 (77.5%) 700.4Mb
>Q15 68951 (29.5%) 247.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 29.6 (10)
2 29.3 (10)
3 28.5 (10)
4 28.4 (10)
5 28.4 (21)
Top 5 longest reads and their mean basecall quality score
1 98675 (14.2)
2 97915 (13.3)
3 87255 (12.2)
4 87040 (14.8)
5 83657 (15.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot