NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 896.9
Mean read quality 10.9
Median read length 677.0
Median read quality 12.2
Number of reads 72,979.0
Read length N50 1,686.0
STDEV read length 921.9
Total bases 65,454,912.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 72537 (99.4%) 65.4Mb
>Q7 71235 (97.6%) 65.2Mb
>Q10 58562 (80.2%) 53.8Mb
>Q12 38696 (53.0%) 32.1Mb
>Q15 10433 (14.3%) 1.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 37.5 (16)
2 37.0 (16)
3 36.1 (16)
4 35.8 (16)
5 35.3 (17)
Top 5 longest reads and their mean basecall quality score
1 8594 (12.3)
2 8374 (10.6)
3 8052 (12.9)
4 7892 (13.0)
5 7544 (12.2)

Summary statistics after filtering

General summary
Mean read length 899.7
Mean read quality 10.9
Median read length 682.0
Median read quality 12.2
Number of reads 72,747.0
Read length N50 1,687.0
STDEV read length 922.0
Total bases 65,451,121.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 72305 (99.4%) 65.4Mb
>Q7 71003 (97.6%) 65.2Mb
>Q10 58330 (80.2%) 53.8Mb
>Q12 38464 (52.9%) 32.1Mb
>Q15 10201 (14.0%) 1.4Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.6 (22)
2 32.6 (21)
3 31.4 (36)
4 31.0 (23)
5 30.8 (46)
Top 5 longest reads and their mean basecall quality score
1 8594 (12.3)
2 8374 (10.6)
3 8052 (12.9)
4 7892 (13.0)
5 7544 (12.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot