NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 965.8
Mean read quality 10.8
Median read length 772.5
Median read quality 12.1
Number of reads 8,668.0
Read length N50 1,821.0
STDEV read length 945.0
Total bases 8,371,542.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 8619 (99.4%) 8.4Mb
>Q7 8489 (97.9%) 8.3Mb
>Q10 6785 (78.3%) 6.7Mb
>Q12 4444 (51.3%) 3.8Mb
>Q15 1358 (15.7%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 35.6 (16)
2 34.5 (16)
3 34.4 (16)
4 34.4 (16)
5 34.1 (17)
Top 5 longest reads and their mean basecall quality score
1 6122 (11.6)
2 6078 (11.0)
3 5979 (11.6)
4 5611 (13.6)
5 5253 (7.7)

Summary statistics after filtering

General summary
Mean read length 969.4
Mean read quality 10.8
Median read length 784.0
Median read quality 12.1
Number of reads 8,635.0
Read length N50 1,821.0
STDEV read length 945.0
Total bases 8,370,999.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 8586 (99.4%) 8.4Mb
>Q7 8456 (97.9%) 8.3Mb
>Q10 6752 (78.2%) 6.7Mb
>Q12 4411 (51.1%) 3.8Mb
>Q15 1325 (15.3%) 0.1Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.2 (20)
2 30.0 (16)
3 30.0 (16)
4 29.9 (16)
5 29.9 (17)
Top 5 longest reads and their mean basecall quality score
1 6122 (11.6)
2 6078 (11.0)
3 5979 (11.6)
4 5611 (13.6)
5 5253 (7.7)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot