NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 731.9
Mean read quality 10.0
Median read length 408.0
Median read quality 11.3
Number of reads 3,050.0
Read length N50 1,486.0
STDEV read length 749.4
Total bases 2,232,250.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 3034 (99.5%) 2.2Mb
>Q7 2971 (97.4%) 2.2Mb
>Q10 1990 (65.2%) 1.7Mb
>Q12 1232 (40.4%) 0.9Mb
>Q15 362 (11.9%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 34.3 (16)
2 33.7 (16)
3 33.4 (16)
4 32.7 (16)
5 32.7 (17)
Top 5 longest reads and their mean basecall quality score
1 5046 (10.9)
2 4269 (10.8)
3 4151 (11.1)
4 4036 (13.0)
5 3847 (8.0)

Summary statistics after filtering

General summary
Mean read length 734.0
Mean read quality 10.0
Median read length 412.0
Median read quality 11.2
Number of reads 3,041.0
Read length N50 1,486.0
STDEV read length 749.5
Total bases 2,232,104.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 3025 (99.5%) 2.2Mb
>Q7 2962 (97.4%) 2.2Mb
>Q10 1981 (65.1%) 1.7Mb
>Q12 1223 (40.2%) 0.9Mb
>Q15 353 (11.6%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 30.0 (17)
2 29.9 (16)
3 29.0 (16)
4 28.8 (16)
5 28.7 (16)
Top 5 longest reads and their mean basecall quality score
1 5046 (10.9)
2 4269 (10.8)
3 4151 (11.1)
4 4036 (13.0)
5 3847 (8.0)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot