NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 1,811.6
Mean read quality 10.8
Median read length 1,725.0
Median read quality 12.0
Number of reads 19,038.0
Read length N50 2,249.0
STDEV read length 1,102.0
Total bases 34,490,136.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 19022 (99.9%) 34.5Mb
>Q7 18912 (99.3%) 34.5Mb
>Q10 15307 (80.4%) 28.3Mb
>Q12 9454 (49.7%) 17.1Mb
>Q15 667 (3.5%) 0.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.9 (16)
2 33.1 (20)
3 32.9 (18)
4 32.6 (16)
5 32.2 (16)
Top 5 longest reads and their mean basecall quality score
1 8912 (11.1)
2 8691 (12.7)
3 8472 (8.9)
4 8461 (12.0)
5 7995 (12.6)

Summary statistics after filtering

General summary
Mean read length 1,812.0
Mean read quality 10.8
Median read length 1,725.0
Median read quality 12.0
Number of reads 19,034.0
Read length N50 2,249.0
STDEV read length 1,101.8
Total bases 34,490,070.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 19018 (99.9%) 34.5Mb
>Q7 18908 (99.3%) 34.5Mb
>Q10 15303 (80.4%) 28.3Mb
>Q12 9450 (49.6%) 17.1Mb
>Q15 663 (3.5%) 0.6Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.1 (20)
2 29.9 (16)
3 29.7 (16)
4 29.6 (16)
5 29.5 (17)
Top 5 longest reads and their mean basecall quality score
1 8912 (11.1)
2 8691 (12.7)
3 8472 (8.9)
4 8461 (12.0)
5 7995 (12.6)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot