NanoPlot statistics report

NanoPlot reports

Summary statistics prior to filtering

General summary
Mean read length 720.8
Mean read quality 9.7
Median read length 404.0
Median read quality 10.8
Number of reads 4,246.0
Read length N50 1,408.0
STDEV read length 760.6
Total bases 3,060,360.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 4220 (99.4%) 3.1Mb
>Q7 4131 (97.3%) 3.0Mb
>Q10 2554 (60.2%) 2.1Mb
>Q12 1482 (34.9%) 1.1Mb
>Q15 406 (9.6%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 33.5 (16)
2 32.7 (16)
3 32.6 (16)
4 31.9 (31)
5 31.0 (16)
Top 5 longest reads and their mean basecall quality score
1 8107 (8.9)
2 6492 (9.6)
3 4946 (12.5)
4 4759 (11.5)
5 4704 (13.2)

Summary statistics after filtering

General summary
Mean read length 721.8
Mean read quality 9.7
Median read length 404.5
Median read quality 10.8
Number of reads 4,240.0
Read length N50 1,408.0
STDEV read length 760.7
Total bases 3,060,264.0
Number, percentage and megabases of reads above quality cutoffs
>Q5 4214 (99.4%) 3.1Mb
>Q7 4125 (97.3%) 3.0Mb
>Q10 2548 (60.1%) 2.1Mb
>Q12 1476 (34.8%) 1.1Mb
>Q15 400 (9.4%) 0.0Mb
Top 5 highest mean basecall quality scores and their read lengths
1 31.9 (31)
2 29.7 (16)
3 29.5 (16)
4 29.2 (19)
5 29.2 (18)
Top 5 longest reads and their mean basecall quality score
1 8107 (8.9)
2 6492 (9.6)
3 4946 (12.5)
4 4759 (11.5)
5 4704 (13.2)

Plots

Weighted histogram of read lengths

Weighted histogram of read lengths after log transformation

Non weighted histogram of read lengths

Non weighted histogram of read lengths after log transformation

Yield by length

Read lengths vs Average read quality plot using dots

Read lengths vs Average read quality kde plot